cleaved caspase 3 Search Results


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cleaved caspase 3  (Novus Biologicals)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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anti cleaved caspase 3  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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d3e9  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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signalstain cleaved caspase 3 ihc detection kit  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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immunosorbent assay elisa kit  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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rabbit anti cleaved caspase 3  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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cleaved caspase 3  (Cell Signaling Technology Inc)
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(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, <t>cleaved</t> <t>CASPASE-3</t> and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.
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Image Search Results


(A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, cleaved CASPASE-3 and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.

Journal: Experimental Animals

Article Title: Acyl-CoA thioesterase 1 (ACOT1) overexpression alleviates heart failure by inhibiting oxidative stress and cardiomyocyte apoptosis through the Kelch-like ECH-associated protein1-NF-E2-related factor2 (KEAP1-NRF2) pathway

doi: 10.1538/expanim.24-0129

Figure Lengend Snippet: (A) The levels of MDA and GSH and the activity of SOD in myocardial tissues were analyzed by ELISA. (B) The protein levels and the quantification results of cleaved PARP, cleaved CASPASE-3 and cleaved CASPASE-9 in myocardial tissues. (C) ROS in myocardial tissues were detected by DHE staining (n=6). The fluorescence intensity was calculated to evaluate ROS levels. bar=50 µ m. # P <0.05, ## P <0.01, ### P <0.001. n=6. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.

Article Snippet: The following antibodies were used: cleaved CASPASE-3 (Affinity, Cincinnati, OH, USA, #AF7022) diluted in 1:1,000; cleaved CASPASE-9 (Affinity, #AF5240) diluted in 1:1,000; cleaved PARP (ABclonal, Wuhan, China, #A22535) diluted in 1:1,000; ACOT1 (Novus Biologicals, Littleton, CO, USA, #NBP3-10843) diluted in 1:500; KEAP1 (Affinity, #AF5266) diluted in 1:1,000; NRF2 (Affinity, #AF0639) diluted in 1:1,000; Histone H3 (Gene Tex, Alton Parkway Irvine, CA, USA, #GTX122148) diluted in 1:5,000; GAPDH (Proteintech, Wuhan, China, #60004-1-Ig) diluted in 1:10,000; Goat Anti-Rabbit IgG/HRP (Solarbio, #SE134) diluted in 1:3,000; Goat Anti-Mouse IgG/HRP (Solarbio, #SE131) diluted in 1:3,000.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Staining, Fluorescence, Plasmid Preparation, Over Expression

(A) Protein level and the quantification of ACOT1 in HL-1 cells with ACOT1 overexpression. (B) cell viability of HL-1 cells with hypoxia treatment. (C) The levels of MDA and GSH and the activity of SOD in cells. (D) The protein levels of cleaved PARP, cleaved CASPASE-3 and cleaved CASPASE-9 in cells and the quantification results. (E) TUNEL staining of HL-1 cells and the positive cell quantification, bar=100 µ m. (F) ROS levels in HL-1 cells were detected by flow cytometry, MFI: mean fluorescence intensity. ## P <0.01, ### P <0.001. n=3. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.

Journal: Experimental Animals

Article Title: Acyl-CoA thioesterase 1 (ACOT1) overexpression alleviates heart failure by inhibiting oxidative stress and cardiomyocyte apoptosis through the Kelch-like ECH-associated protein1-NF-E2-related factor2 (KEAP1-NRF2) pathway

doi: 10.1538/expanim.24-0129

Figure Lengend Snippet: (A) Protein level and the quantification of ACOT1 in HL-1 cells with ACOT1 overexpression. (B) cell viability of HL-1 cells with hypoxia treatment. (C) The levels of MDA and GSH and the activity of SOD in cells. (D) The protein levels of cleaved PARP, cleaved CASPASE-3 and cleaved CASPASE-9 in cells and the quantification results. (E) TUNEL staining of HL-1 cells and the positive cell quantification, bar=100 µ m. (F) ROS levels in HL-1 cells were detected by flow cytometry, MFI: mean fluorescence intensity. ## P <0.01, ### P <0.001. n=3. EP: empty plasmid; ACOT1 oe : ACOT1 overexpression.

Article Snippet: The following antibodies were used: cleaved CASPASE-3 (Affinity, Cincinnati, OH, USA, #AF7022) diluted in 1:1,000; cleaved CASPASE-9 (Affinity, #AF5240) diluted in 1:1,000; cleaved PARP (ABclonal, Wuhan, China, #A22535) diluted in 1:1,000; ACOT1 (Novus Biologicals, Littleton, CO, USA, #NBP3-10843) diluted in 1:500; KEAP1 (Affinity, #AF5266) diluted in 1:1,000; NRF2 (Affinity, #AF0639) diluted in 1:1,000; Histone H3 (Gene Tex, Alton Parkway Irvine, CA, USA, #GTX122148) diluted in 1:5,000; GAPDH (Proteintech, Wuhan, China, #60004-1-Ig) diluted in 1:10,000; Goat Anti-Rabbit IgG/HRP (Solarbio, #SE134) diluted in 1:3,000; Goat Anti-Mouse IgG/HRP (Solarbio, #SE131) diluted in 1:3,000.

Techniques: Over Expression, Activity Assay, TUNEL Assay, Staining, Flow Cytometry, Fluorescence, Plasmid Preparation